Practical Skills in Biomolecular Sciences - Rob Reed - 9780132391153 - Biology - General Biology - Pearson Education Schweiz AG - Der Fachverlag fuer Bildungsmedien - 978-0-1323-9115-3
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Practical Skills in Biomolecular Sciences

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Titel:   Practical Skills in Biomolecular Sciences
Reihe:   Benjamin Cummin
Autor:   Rob Reed / Jonathan Weyers / Allan Jones / David Holmes
Verlag:   Benjamin Cummings
Einband:   Softcover
Auflage:   3
Sprache:   Englisch
Seiten:   568
Erschienen:   Juli 2007
ISBN13:   9780132391153
ISBN10:   0-13-239115-5
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Practical Skills in Biomolecular Sciences

Description

Practical Skills in Biomolecular Science, is an indispensable book for undergraduate students in the life sciences. The book provides useful support at all stages of a degree course and underpins any practical course in biochemistry, biomedical science, genetics, immunology and microbiology. It is also a valuable resource for teachers of biology in colleges and secondary schools.  Laboratory and field studies are essential components of undergraduate training in biomolecular science. Practical work must be fully understood and effectively presented, but many students under-perform because they lack basic laboratory skills. This book, now in its third edition, continues to provide students with easy-to-use guidance for laboratory and field studies, but in addition it now covers broader transferable skills. As a result the new edition provides guidance and support over the entire range of a typical undergraduate course in biochemistry and biomedical science.  


New to this Edition

New material covering:

 

  • Personal Development Planning
  • Learning styles and thinking processes
  • Writing styles
  • Plagiarism
  • Copyright
  • E-Learning
  • Bioethics
  • Digital imaging
  • Eukaroyte cell culture
  • ELISA assays
  • Real-time PCR
  • Guidance on using software to enhance presentations; drawing graphs and tables, and doing statistical analysis.

 

Safety issues are now emphasised using a new ‘Safety’ icon throughout the book. These indicate potential hazards and risks and strategies to avoid them.

 

All references updated, and all web-links checked.

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Table of Contents

List of boxes

Preface

Guided tour

For the student

Acknowledgements

List of abbreviations

 

Study and examination skills

1 The importance of transferable skills

2 Managing your time

3 Working with others 

4 Taking notes from lectures and texts

5 Learning and revising

6 Curriculum options, assessments and exams

7 Preparing your curriculum vitae

 

Information technology and library resources

8 Finding and citing published information

9 Evaluating information

10 Using online resources

11 Bioinformatics - Internet resources

12 Using spreadsheets

13 Word processors, databases and other packages

 

Communicating information

14 Organising a poster display

15 Giving a spoken presentation

16 General aspects of scientific writing

17 Writing essays

18 Reporting practical and project work

19 Writing literature surveys and reviews

 

Fundamental laboratory techniques

20 Your approach to practical work

21 Health and safety

22 Working with liquids

23 Basic laboratory procedures

24 Principles of solution chemistry

25 pH and buffer solutions

26 Introduction to microscopy

27 Setting up and using a light microscope

 

The investigative approach

28 Making and recording measurements

29 SI units and their use

30 Scientific method and design of experiments

31 Project work

 

Handling cells and tissues

32 Sterile technique and microbial culture

33 Isolating, identifying and naming microbes

34 Working with animal and plant tissues and cells

35 Culture systems and growth measurement

36 Homogenisation and fractionation of cells and tissues

 

Analytical techniques

37 Calibration and its application to quantitative analysis

38 Immunological methods

39 Radioactive isotopes and their uses

40 Light measurement

41 Basic spectroscopy

42 Advanced spectroscopy and spectrometry

43 Centrifugation

44 Chromatography - separation methods

45 Chromatography - detection and analysis

46 Principles and practice of electrophoresis

47 Advanced electrophoretic techniques

48 Electroanalytical techniques

 

Assaying biomolecules and studying metabolism

49 Analysis of biomolecules: fundamental principles

50 Assaying amino acids, peptides and proteins

51 Assaying lipids

52 Assaying carbohydrates

53 Assaying nucleic acids and nucleotides

54 Protein purification

55 Enzyme studies

56 Membrane transport processes

57 Photosynthesis and respiration

 

Genetics

58 Mendelian genetics

59 Bacterial and phage genetics

60 Molecular genetics I - fundamental principles

61 Molecular genetics II - PCR and related applications

62 Molecular genetics III - genetic engineering techniques

 

Analysis and presentation of data

63 Using graphs

64 Presenting data in tables

65 Hints for solving numerical problems

66 Descriptive statistics

67 Choosing and using statistical tests

 

Answers to study exercises

Index

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Back Cover

"An essential book for all Life Science students – a highly recommended purchase for their degree."

Dr Mark Wilkinson, University of Liverpool

If you are studying within the biomolecular sciences, then this book is an indispensable companion throughout your entire degree programme. It  demonstrates lucidly the laboratory and data analysis skills that you will draw on time and again for the practical aspects of your studies, and also gives you a solid grounding in those wider transferable skills that are increasingly necessary to achieve a higher level of academic success.

This third edition builds on the excellent foundation of its predecessors. It contains a fully updated examination of existing topics and covers even more core skills, including:

  • ELISA assays
  • DNA micro-arrays
  • Digital imaging
  • Eukaroyte cell culture
  • Real-time PCR
  • Bioethics
  • Personal development planning
  • Learning styles
  • Avoiding plagiarism
  • E-learning

§         Thinking processes

§         Use of software to enhance graph/table drawing, statistical analysis and presentations

Key features

  • New 'safety note' feature, highlighting specific hazards and appropriate practical steps to minimise risk
  • Worked examples and ‘how to’ boxes that set out the essential procedures in a step-by-step manner
  • Key points highlighting critical features of methodology
  • Study exercises in each chapter to reinforce learning
  • Use of margin tips, definitions and illustrations throughout

 

Rob Reed is Professor of Biomedical Sciences at Central Queensland University;  David Holmes is Head of the Division of Biomedical Sciences at Northumbria University; Jonathan Weyers is Director of Quality Assurance at the University of Dundee; and Allan Jones is Senior Lecturer in Biology and Life Sciences at the University of Dundee.

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