|Techniques for Microbiology||
Techniques for Microbiology
|52.50||approx. 7-9 days|
Lammert's approach is visual and incorporates “voice balloons” that keep the student focused on the process described. The techniques are those that will be used frequently for studying microbes in the laboratory, and include those identified by the American Society for Microbiology in its recommendations for the Microbiology Laboratory Core Curriculum (recommendations in which the author participated).
• Unique, full-color artwork with accompanying step-by-step instructions
- Helps students visualize techniques as an integrated process.
• Brief presentation of techniques:
- The concise and easy-to-follow presentations of techniques in the handbook will make it more likely that the student will preview the technique before the lab period.
- Concise and illustrative presentation will appeal to visual learners.
• Photographs that show the outcomes of enzyme-catalyzed reactions - Appear with the test procedure rather than in a separate grouping.
• Portable 6” x 9” trim size and spiral binding -for students' convenience and effective use on the lab bench surface.
• Suitable as a supplement to an in-house or commercial manual.
Unit I - Safety first!
Safety in the microbiology laboratory
Unit II - Culturing bacteria and aseptic techniques
Preparing culture media
Aseptic transfer of bacteria
Adjusting the gas burner
Preparing bacterial cultures in broth tubes and on agar slants
Transfer colony from plate to broth and to agar slant
Isolation of bacteria
Preparing a pour plate
Preparing a streak plate
Preparing a spread plate
Characteristic features of bacterial growth in broth and on agar
Maintenance and storage of stock cultures
Culturing anaerobic bacteria
Unit III - Visualizing bacteria
Effective use and responsible care of the light microscope
Measuring microscopic cells
Preparing a hanging-drop slide
Preparing a bacterial smear
Preparing a simple stain
Preparing a Gram stain
Preparing an acid-fast stain
Preparing a negative stain
Preparing a capsule stain
Preparing an endospore stain
Unit IV - Enzyme-based tests for the identification of bacteria
Hydrolytic (digestive) enzymes
Lipid hydrolysis (tributyrin and spirit blue agar)
Utilization of carbohydrates
Fermentation of carbohydrates (Durham tube)
Methyl red test (mixed fermentation)
Voges-Proskauer test (butanediol fermentation)
Oxidation-fermentation (O-F) glucose test
Degradation of amino acids
Triple sugar iron (TSI) test
Litmus milk reactions
Selective and/or differential media
Eosin methylene blue (EMB) agar
Phenylethyl alcohol (PEA) agar
Unit V - Counting microbes
Direct microscopic counting with the Petroff-Hausser chamber
Preparing a standard plate count of bacteria
Using Turbidemetry to estimate cell density
Plaque assay for determining bacteriophage titer
Unit VI - Measuring effectiveness of antibacterial chemicals
Kirby-Bauer method for sensitivity of bacteria to antibacterial
Evaluating antibacterial chemicals: The disk-diffusion