Description

Containing 57 thoroughly class-tested and easily customizable exercises, Laboratory Experiments in Microbiology, Tenth Edition, provides engaging labs with instruction on performing basic microbiology techniques and applications for undergraduate students in diverse areas, including the biological sciences, allied health sciences, agriculture, environmental science, nutrition, pharmacy, and various pre-professional programs. The perfect companion to Tortora/Funke/Case’s Microbiology: An Introduction or any introductory microbiology text, the Tenth Edition features an updated art program and a full-color design, integrating valuable micrographs throughout each exercise. Additionally, many of the illustrations have been re-rendered in a modern, realistic, three-dimensional style to better visually engage students. Laboratory Reports for each exercise have been enhanced with new Clinical Applications questions, as well as questions relating to Hypotheses or Expected Results. Experiments have been refined throughout the manual and the Tenth Edition includes an extensively revised exercise on transformation in bacteria using pGLO to introduce students to this important technique.

Features

• Comprehensive coverage of core microbiology topics including exercises in the areas of genetics and immunology.
• Laboratory Reports close out each exercise and help students learn to collect, record, and present data systematically.
• Consistently organized exercises include objectives; background definitions and explanations; lists of materials, supplies, and cultures needed; techniques required, with cross-references to previous exercises; step-by-step instructions; and space to record results.
• Questions embedded in the Background, Procedures, and Lab Report sections emphasize critical-thinking skills, requiring students to recall and apply information through analysis and logical reasoning.
• A Conclusions section consisting of a series of investigative questions is consistently included in the Lab Report of each exercise, encouraging students to closely analyze and understand their results from each laboratory exercise.
• 100 photographs in full color, and numerous diagrams and drawings assist the student in visualizing techniques and interpreting results.
• An updated Preparation Guide written by the authors has specific instructions (i.e., recipes, materials) for preparing each lab, safety guidelines for handling blood and other bodily fluids, and page references to Tortora, Funke, and Case’s Microbiology: An Introduction, Eleventh edition.
• Tortora/Funke/Case, Microbiology: An Introduction and Johnson/Case, Laboratory Experimiments in Microbiology are available in the Pearson Custom Library, allowing instructors to create the perfect text with labs for their course. Select the chapters you need, in the sequence you want and delete chapters you don’t use: Your students pay only for the materials you choose. To create the perfect text, visit: http://www.pearsonlearningsolutions.com/custom-library/

New to this Edition

• The art program features precise illustrations in full-color. Full-color photographs appear alongside the relevant exercise, allowing students to clarify their experiments and better interpret their results. Twenty-five new photos have been included, and new illustrations have been added to Exercises 9, 11, 25, 26, 27, 29, 30, 40, and 44 to further illustrate and explain complex procedures.
• Expected Results or Hypothesis Section in each exercise’s Laboratory Report encourages students to speculate on the outcome of experimental procedures and to formulate a hypothesis when appropriate.
• Clinical Application questions, which have a clinical basis or are derived from a clinical situation, have been added to each Laboratory Report to help students draw connections between what they learn in lab and allied health careers.
• Prelab questions, multiple choice and true/false, are available online for laboratory instructors' use to ensure their students prepare adequately for each assigned laboratory exercise. Pre-lab questions are also available and assignable in MasteringMicrobiology®.
• Exercise on Transformation of Bacteria has been extensively revised to incorporate use of pGLO to introduce students to this important tool used in microbiology.
• Eye-catching full-color design engages the student while highlighting this manual’s trademark clear organization and presentation.

Table of Contents

Introduction

I. MICROSCOPY

1. Use and Care of the Microscope

2. Examination of Living Microorganisms

II. Handling Bacteria

3. Microbes in the Environment

4. Transfer of Bacteria: Aseptic Techniques

III. STAINING METHODS

5. Preparation of Smears and Simple Staining

6. Negative Staining

7. Gram Staining

8. Acid-Fast Staining

9. Structural Stains (Endospore, Capsule, Flagella)

10. Morphologic Unknown

IV. CULTIVATION OF BACTERIA  

11. Isolation of Bacteria by Dilution Techniques

12. Special Media for Isolating Bacteria

V. MICROBIAL METABOLISM

13. Carbohydrate Catabolism

14. Fermentation

15. Protein Catabolism, Part 1

16. Protein Catabolism, Part 2

17. Respiration

18. Unknown Identification and Bergey’s Manual

VI. MICROBIAL GROWTH

19. Oxygen and the Growth of Bacteria

20. Determination of a Bacterial Growth Curve: The Role of Temperature

21. Biofilms  

VII. CONTROL OF MICROBIAL GROWTH

22. Physical Methods of Control: Heat

23. Physical Methods of Control: Ultraviolet Radiation

24. Chemical Methods of Control: Disinfectants and Antiseptics

25. Chemical Methods of Control: Antimicrobial Drugs

26. Effectiveness of Hand Scrubbing

VIII. MICROBIAL GENETICS

27. Regulation of Gene Expression

28. Isolation of Bacterial Mutants

29. Transformation of Bacteria

30. DNA Fingerprinting

31. Genetic Engineering

32. Ames Test for Detecting Possible Chemical Carcinogens

IX. THE MICROBIAL WORLD

33. Fungi: Yeasts and Molds

34. Phototrophs: Algae and Cyanobacteria

35. Protozoa

36. Parasitic Helminths

X. VIRUSES

37. Isolation and Titration of Bacteriophages

38. Plant Viruses

XI. INTERACTION OF MICROBE AND HOST

39. Epidemiology

40. Koch’s Postulates

XII. IMMUNOLOGY

41. Innate Immunology

42.  Agglutination Reactions: Slide Agglutination

43. Agglutination Reactions: Microtiter Agglutination

44. ELISA Technique

XIII. MICROORGANISMS AND DISEASE

45. Bacteria of the Skin

46. Bacteria of the Respiratory Tract

47. Bacteria of the Mouth

48. Bacteria of the Gastrointestinal Tract

49. Bacteria of the Genitourinary Tract

50. Identification of an Unknown from a Clinical Sample

51. Rapid Identification Methods

XIV. MICROBIOLOGY AND THE ENVIRONMENT

52. Microbes in Water: Multiple-Tube Technique

53. Microbes in Water: Membrane Filter Technique

54. Microbes in Food: Contamination

55. Microbes Used in the Production of Foods

56. Microbes in Soil: The Nitrogen and Sulfur Cycles

57. Microbes in Soil: Bioremediaton

APPENDICES

A. Pipetting

B. Dilution Techniques and Calculations

C. Use of the Spectrophotometer

D. Graphing

E. Use of the Dissecting Membrane

F. Use of the Membrane Filter

G. Electrophoresis

H. Keys to Bacteria

Index