Description

For courses in Microbiology Lab and Nursing and Allied Health Microbiology Lab

A Flexible Approach to the Modern Microbiology Lab

Easy to adapt for almost any microbiology lab course, this versatile, comprehensive, and clearly written manual is competitively priced and can be paired with any undergraduate microbiology text. Known for its thorough coverage, straightforward procedures, and minimal equipment requirements, the Eleventh Edition incorporates current safety protocols from governing bodies such as the EPA, ASM, and AOAC. The new edition also includes alternate organisms for experiments for easy customization in Biosafety Level 1 and 2 labs. New lab exercises have been added on Food Safety and revised experiments, and include options for alternate media, making the experiments affordable and accessible to all lab programs. Ample introductory material, engaging clinical applications, and laboratory safety instructions are provided for each experiment along with easy-to-follow procedures and flexible lab reports with review and critical thinking questions.

Features

• NEW! A new experiment on the Propagation of Isolated Bacteriophage Cultures has been added to the Eleventh Edition. This experiment (39) guides students to isolate bacteriophages for genetic manipulation. The genetic manipulation of bacteriophages is an important technique in current clinical research as a possible way to treat antibiotic-resistant bacterial infections.
• NEW! Biosafety Levels (BSLs) have been added to the Eleventh Edition to alert students to appropriate safety techniques. The organisms within this book are mostly BSL-1 with some optional BSL-2 organisms. The Eleventh Edition also reflects the most up to date safety protocols from governing bodies such as the EPA, ASM, and AOAC, better preparing students for professional lab work.
• NEW! Tips for Success appear in select experiments and draw attention to common mistakes and stumbling blocks in the lab. Each tip explains why specific techniques are necessary to yield accurate results, helping guide students on crucial procedural steps and maximizing the available lab media and time.
• NEW! Revised experiments include options for alternate media, making the experiments affordable and accessible to all sizes of lab programs. Experiment 60 has been revised to focus on the normal microbiota of human skin and the importance of hand washing.
• NEW! Easy-to-adapt Lab Reports include blank spaces or options for “alternate organisms” for easy customization with organisms that are readily available.
• MasteringMicrobiology Lab-specific Assignments are available within each student’s subscription for the Pearson online homework and assessment program. To help students prepare for each class and get the most out of lab time, instructors may assign:
  • Pre-lab quizzes for each of the 72 experiments in the Cappuccino/Welsh Lab Manual; these consist of 10 multiple-choice questions with personalized feedback for wrong answers. Each question includes a Bloom’s taxonomy rating.
  • MicroLab Tutor Coaching Activities combine live-action technique videos and molecular animations, when appropriate, with assessment and answer-specific feedback to coach students in how to correctly perform each lab procedure and interpret/analyze different lab results.
  • Lab Technique Videos give students an opportunity to see techniques performed correctly and quiz themselves on lab procedures both before and after lab time. New Lab Technique videos demonstrate The Scientific Method and How to Write a Lab Report.
  • MicroLab Practical Activities assess students’ ability to interpret and analyze important lab tests, procedures, and results before their Lab Practical.
• Clinical Applications are integrated into each lab to help students understand why they’re learning each technique and make the connection between lab, lecture, and possible future careers as nurses or in other allied health fields.
• The comprehensive coverage of core microbiology topics includes experiments in the areas of genetics, immunology, and biotechnology.
• A wide range of experiments progressing from simple to complex enable instructors to tailor their laboratory classes to the topics they wish to cover.
• Experiments use the most common and affordable laboratory materials, designed to accommodate any lab.
• Over 90 photographs in full color and numerous illustrations appear directly alongside the experiments, helping students visualize techniques and expected results.

New to this Edition

• A new experiment on the Propagation of Isolated Bacteriophage Cultures has been added to the Eleventh Edition. This experiment (39) guides students to isolate bacteriophages for genetic manipulation. The genetic manipulation of bacteriophages is an important technique in current clinical research as a possible way to treat antibiotic-resistant bacterial infections.
• Biosafety Levels (BSLs) have been added to the Eleventh Edition to alert students to appropriate safety techniques. The organisms within this book are mostly BSL-1 with some optional BSL-2 organisms. The Eleventh Edition also reflects the most up to date safety protocols from governing bodies such as the EPA, ASM, and AOAC, better preparing students for professional lab work.
• Tips for Success appear in select experiments and draw attention to common mistakes and stumbling blocks in the lab. Each tip explains why specific techniques are necessary to yield accurate results, helping guide students on crucial procedural steps and maximizing the available lab media and time.
• Revised experiments include options for alternate media, making the experiments affordable and accessible to all sizes of lab programs. Experiment 60 has been revised to focus on the normal microbiota of human skin and the importance of hand washing.
• Easy-to-adapt Lab Reports include blank spaces or options for “alternate organisms” for easy customization with organisms that are readily available.

Table of Contents

• Part 1 Basic Laboratory Techniques for Isolation, Cultivation, and Cultural Characterization of Microorganisms
• 1. Culture Transfer Techniques
• 2. Techniques for Isolation of Pure Cultures
• 3. Cultural Characteristics of Microorganisms
• 4. Microscopic Examination of Stained Cell Preparations
• 5. Microscopic Exami­na­tion of Living Microorganisms Using a Hanging-Drop Preparation or a Wet Mount
• Part 3 Bacterial Staining
• 6. Preparation of Bacterial Smears
• 7. Simple Staining
• 8. Negative Staining
• 9. Gram Stain
• 10. Acid-Fast Stain
• 11. Differential Staining for Visualization of Bacterial Cell Structures
• Part 4 Cultivation of Microorganisms: Nutritional and Physical Requirements, and Enumeration of Microbial Populations
• 12. Nutritional Requirements: Media for the Routine Cultivation of Bacteria
• 13. Use of Differential, Selective, and Enriched Media
• 14. Physical Factors: Temperature
• 15. Physical Factors: pH of the Extracellular Environment
• 16. Physical Factors: Atmospheric Oxygen Requirements
• 17. Techniques for the Cultivation of Anaerobic Microorganisms
• 18. Serial Dilution—Agar Plate Procedure to Quantitate Viable Cells
• 19. The Bacterial Growth Curve
• Part 5 Biochemical Activities of Microorganisms
• 20. Extracellular Enzymatic Activities of Microorganisms
• 21. Carbohydrate Fermentation
• 22. Triple Sugar—Iron Agar Test
• 23. IMViC Test
• 24. Hydrogen Sulfide Test
• 25. Urease Test
• 26. Litmus-Milk Reactions
• 27. Nitrate Reduction Test
• 28. Catalase Test
• 29. Oxidase Test
• 30. Utilization of Amino Acids
• 31. Genus Identification of Unknown Bacterial Cultures
• Part 6 The Protozoa
• 32. Free-Living Protozoa
• 33. Parasitic Protozoa
• Part 7 The Fungi
• 34. Cultivation and Morphology of Molds
• 35. Yeast Morphology, Cultural Characteristics, and Reproduction
• 36. Identification of Unknown Fungi
• Part 8 The Viruses
• 37. Cultivation and Enumeration of Bacteriophages
• 38. Isolation of Coliphages from Raw Sewage
• 39. Propagation of Isolated Bacteriophage Cultures
• Part 9 Physical and Chemical Agents for the Control of Microbial Growth
• 40. Physical Agents of Control: Moist Heat
• 41. Physical Agents of Control: Electromagnetic Radiations
• 42. Chemical Agents of Control: Chemotherapeutic Agents
• 43. Determination of Penicillin Activity in the Presence and Absence of Penicillinase
• 44. Chemical Agents of Control: Disinfectants and Antiseptics
• Part 10 Microbiology of Food
• 45. Microbiological Analysis of Food Products: Bacterial Count
• 46. Microbial Fermentation
• Part 11 Microbiology of Water
• 47. Standard Qualitative Analysis of Water
• 48. Quantitative Analysis of Water: Membrane Filter Method
• Part 12 Microbiology of Soil
• 49. Microbial Populations in Soil: Enumeration
• 50. Isolation of Antibiotic-Producing Microorganisms and Determination of Antimicrobial Spectrum of Isolates
• 51. Isolation of Pseudomonas Species by Means of the Enrichment Culture Technique